This guidance document includes an updated summary of diagnostic PCR and serology together with detailed information on isolation, culture, identification and epidemiological typing of B. pertussis to help users choose the best methods within the local technical and financial provisions.
The Protocol is targeted at the national public health reference laboratories to guide the susceptibility testing needed for EU surveillance and the reporting to ECDC.
The seroincidence calculator utilises the combination of serum antibody levels (lgG, lgM, and lgA) at a given point in time to estimate the time since seroconversion, which in turn gives an estimate of annual 'force of infection' in the tested population.
This publication aims to provide EU/EEA data providers with a single protocol for performing multiple-locus variable-number tandem repeat analysis (MLVA) of Salmonella enterica serotype Enteritidis (S. Enteritidis) and thereby ensure interlaboratory comparability of the generated results using capillary electrophoresis (CE).
This report describes many of the aspects that should be taken into account. It systematically covers the entire process from sample provision and sequencing to data analysis and collaboration with other organisations.
This protocol for harmonised monitoring of antimicrobial resistance in Salmonella and Campylobacter from human isolates aims to increase the quality and comparability of antimicrobial resistance data collected at the EU level from different Member States. It is primarily targeted to the National Public Health Reference Laboratories to guide the susceptibility testing needed for EU surveillance and the reporting to ECDC.
This report describes the standardised laboratory protocol for molecular subtyping of Salmonella enterica serotype Typhimurium. It is based on the 5-locus multiple-locus variable-number tandem repeat analysis (MLVA) method.