Laboratory standard operating procedure for MLVA of Salmonella enterica serotype Typhimurium
This technical document provides a single protocol for performing MLVA of S. Typhimurium and thereby ensure inter-laboratory comparability of the generated results using capillary electrophoresis.
The 5-locus MLVA method has been widely used in Europe in recent years. European laboratories have agreed to use a new nomenclature based on the actual number of repeat units in each locus, therefore independent of the equipment used for fragment analysis. This will significantly enhance the possibility of detecting international foodborne outbreaks of Salmonella.
ECDC’s technical document provides a single protocol for performing MLVA of S. Typhimurium and ensure inter-laboratory comparability of the generated results using capillary electrophoresis. ECDC supported the development of the protocol aiming at establishing MLVA for typing of Salmonella Typhimurium in public health laboratories and selected veterinary laboratories in Europe. The protocol will enable implementation of the method so that results are comparable between laboratories.
European surveillance of Clostridioides (Clostridium) difficile infections - surveillance protocol version 2.4
17 Dec 2019 - ECDC publishes a protocol developed for the surveillance of Clostridioides (Clostridium) difficile infections (CDI) to address the lack of standardised surveillance of CDI in EU Member States.
Managing heterogeneity when pooling data from different surveillance systems
21 Oct 2019 - This report addresses the heterogeneity that arises from pooling data from different surveillance systems and provides statistical and procedural approaches to minimise or remove its impact. The aim is to support public health specialists and researchers in answering key research and policy questions using available European data to the fullest possible extent.
Options for national testing and surveillance for hepatitis E virus in the EU/EEA - Operational guidance
5 Sep 2019 - This operational guidance, developed by ECDC in collaboration with its hepatitis E virus (HEV) expert group, offers options on the implementation or adjustment of HEV surveillance at a national level.