Laboratory standard operating procedure for MLVA of Salmonella enterica serotype Typhimurium
This technical document provides a single protocol for performing MLVA of S. Typhimurium and thereby ensure inter-laboratory comparability of the generated results using capillary electrophoresis.
The 5-locus MLVA method has been widely used in Europe in recent years. European laboratories have agreed to use a new nomenclature based on the actual number of repeat units in each locus, therefore independent of the equipment used for fragment analysis. This will significantly enhance the possibility of detecting international foodborne outbreaks of Salmonella.
ECDC’s technical document provides a single protocol for performing MLVA of S. Typhimurium and ensure inter-laboratory comparability of the generated results using capillary electrophoresis. ECDC supported the development of the protocol aiming at establishing MLVA for typing of Salmonella Typhimurium in public health laboratories and selected veterinary laboratories in Europe. The protocol will enable implementation of the method so that results are comparable between laboratories.
Managing heterogeneity when pooling data from different surveillance systems
21 Oct 2019 - This report addresses the heterogeneity that arises from pooling data from different surveillance systems and provides statistical and procedural approaches to minimise or remove its impact. The aim is to support public health specialists and researchers in answering key research and policy questions using available European data to the fullest possible extent.
Options for national testing and surveillance for hepatitis E virus in the EU/EEA - Operational guidance
5 Sep 2019 - This operational guidance, developed by ECDC in collaboration with its hepatitis E virus (HEV) expert group, offers options on the implementation or adjustment of HEV surveillance at a national level.
Expert consensus protocol on carbapenem resistance detection and characterisation for the survey of carbapenem- and/or colistin-resistant Enterobacteriaceae
14 Jan 2019 - This expert consensus protocol for carbapenem resistance detection and characterisation was jointly developed by the EURGenCCRE consortium, the scientific advisory board for EURGen-Net and ECDC to agree upon the protocol for phenotypic and genotypic carbapenem resistance detection and confirmation to be used for the CCRE survey.