The seroincidence calculator utilises the combination of serum antibody levels (lgG, lgM, and lgA) at a given point in time to estimate the time since seroconversion, which in turn gives an estimate of annual 'force of infection' in the tested population.
Laboratories should receive clinical and epidemiological information for establishing their investigation strategy, including date of onset of illness, travel history (date and locations), past flaviviral immunisation records and pregnancy status.