This guidance document includes an updated summary of diagnostic PCR and serology together with detailed information on isolation, culture, identification and epidemiological typing of B. pertussis to help users choose the best methods within the local technical and financial provisions.
ECDC promotes the performance of external quality assessment (EQA) schemes, in which laboratories are sent simulated clinical specimens or bacterial isolates for testing by routine or reference laboratory methods. EQA schemes, or laboratory proficiency testing, provide information about the accuracy of different characterisation and typing methods as well as antimicrobial susceptibility testing (AST) and the sensitivity of the methods in place to detect a certain pathogen or novel resistance patterns.
ECDC is addressing the harmonisation and improvement of pertussis diagnosis for surveillance and outbreak detection/monitoring in order to assure quality and comparability of data. The guidance and protocol are intended for real-time PCR on DNA extracted from clinical specimens obtained from patients with suspected whooping cough (i.e. Bordetella pertussis or B. parapertussis).
Current childhood vaccination schedules are the result of historical tradition, compliance with provision of health services and national vaccine registration. They have been designed on the basis of different needs related to how the healthcare system – but also the education system – is organised at national level.