This guidance document includes an updated summary of diagnostic PCR and serology together with detailed information on isolation, culture, identification and epidemiological typing of B. pertussis to help users choose the best methods within the local technical and financial provisions.
The Middle East respiratory syndrome coronavirus (MERS-CoV) was first discovered in 2012 in a patient who died from severe respiratory disease. Since then, and as of 16 January 2020, 2 521 cases have been reported worldwide, including 919 deaths (crude case fatality 36%). Most cases have been reported from the Arabian Peninsula (and around 84% specifically from Saudi Arabia), and all cases elsewhere had a travel history to the Arabian Peninsula or were a contact of someone who had a travel history to the region.
ECDC is addressing the harmonisation and improvement of pertussis diagnosis for surveillance and outbreak detection/monitoring in order to assure quality and comparability of data. The guidance and protocol are intended for real-time PCR on DNA extracted from clinical specimens obtained from patients with suspected whooping cough (i.e. Bordetella pertussis or B. parapertussis).
Current childhood vaccination schedules are the result of historical tradition, compliance with provision of health services and national vaccine registration. They have been designed on the basis of different needs related to how the healthcare system – but also the education system – is organised at national level.