External quality assurance scheme for typing of verocytotoxin-producing E. coli (VTEC)
This report presents the results of the second round of the external quality assurance scheme for typing of verocytotoxin-producing Escherichia coli. Conducted between December 2009 and June 2010, it included O:H serotyping, detection and typing of eae, vtx1, vtx2 and ehxA genes, phenotypic detection of verocytotoxin/shiga toxin-production, fermentation of sorbitol and production of β-glucuronidase and enterohaemolysin for VTEC.
An average of 78% of participating laboratories from EU/EEA countries were able to correctly O:H serotype the ten VTEC strains that were included in the EQA and correct phenotypic characterisation was generally very high.
ECDC’s external quality assurance (EQA) schemes are designed to evaluate the performance of laboratories, to identify areas for improvement in laboratory diagnostics relevant to disease surveillance and to ensure comparability of results between laboratories in EU/EEA countries.
The second EQA scheme for typing of verocytotoxin-producing Escherichia coli (VTEC) was commissioned by ECDC and produced by Statens Serum Institut, Denmark. Forty-five laboratories of the Food- and Waterborne Diseases Network (FWD-Net) from 38 countries participated, of which 23 were from the EU/EEA countries.
The main objective of this EQA scheme was to assess whether typing of verocytotoxin-producing Escherichia coli (VTEC) strains by laboratories within and outside the European Union was carried out uniformly and whether comparable results could be obtained.
The results highlight the importance of genotypic detection, together with the need for improvements in some laboratories regarding the detection methodology used for specific subtypes.
Community engagement and institutional collaboration during outbreaks of Shiga toxin/verocytotoxin-producing Escherichia coli in Ireland
19 Nov 2019 - This country visit report presents the findings of a case study into outbreaks of Shiga toxin/verocytotoxin-producing Escherichia coli (STEC/VTEC) at childcare facilities in Ireland, conducted in November 2018.
Community engagement and institutional collaboration in Iceland during a norovirus outbreak at an outdoor/scout centre (10–15 August 2017)
19 Nov 2019 - This country visit report presents the findings of a case study into an outbreak of norovirus in Iceland that occurred during the period 10–15 August 2017.
A spatial modelling method for vector surveillance
14 Nov 2019 - Vector borne diseases are a specific group of infectious diseases that are a (re-)emerging threat to Europe. One important aspect of preparedness for vector borne diseases is the surveillance of the introduction, establishment and spread of the main disease vectors. ECDC regularly publishes updated vector distribution maps at the NUTS3 level. This document describes a methodology to estimate the vector distribution status for those NUTS3 units for which observations are not yet available . These estimates are produced with spatial modelling techniques, using the currently available distributions to calibrate the modelling process. This document provides an overview of gap analysis procedures, sets out the full methodology, and also provide s details of which methodological components were used with each output provided.