Laboratory standard operating procedure for detecting sporadic Creutzfeldt-Jakob disease using Real- Time Quaking-Induced Conversion (RT-QuIC) assay
This document provides laboratories with a single protocol for producing recombinant full-length hamster prion protein and using it to perform the Real-Time Quaking-Induced Conversion assay (RT-QuIC), which can distinguish sporadic Creutzfeldt-Jakob disease (sCJD) from variant Creutzfeldt-Jakob disease (vCJD).
This protocol describes how to produce recombinant hamster prion protein and use it to perform RT-QuIC. It is based on methods published by the Caughey Group and the National CJD Research and Surveillance Unit at the University of Edinburgh, United Kingdom (NCJDRSU).
Variant Creutzfeldt-Jakob disease
Variant Creutzfeldt-Jakob disease (vCJD) is a relatively new and rare neurological disease, classified as a Transmissible Spongiform Encephalopathy (TSE).Read more