Laboratory standard operating procedure for detecting sporadic Creutzfeldt-Jakob disease using Real- Time Quaking-Induced Conversion (RT-QuIC) assay

This document provides laboratories with a single protocol for producing recombinant full-length hamster prion protein and using it to perform the Real-Time Quaking-Induced Conversion assay (RT-QuIC), which can distinguish sporadic Creutzfeldt-Jakob disease (sCJD) from variant Creutzfeldt-Jakob disease (vCJD).

This protocol describes how to produce recombinant hamster prion protein and use it to perform RT-QuIC. It is based on methods published by the Caughey Group[1] and the National CJD Research and Surveillance Unit at the University of Edinburgh, United Kingdom (NCJDRSU).