Analysis plan for the survey of carbapenem-resistant Enterobacterales 2025 (CRE25 survey)
The survey of carbapenem-resistant Enterobacterales 2025 (CRE25 survey) is the third genomic survey of CRE following the European survey of carbapenemase-producing Enterobacteriaceae (EuSCAPE) in 2013−2014 and the survey of carbapenem- and/or colistin-resistant Enterobacterales (CCRE survey) in 2019. National reference laboratories (NRLs) from the 37 countries that are members of the European Antimicrobial Resistance Genes Surveillance Network (EURGen-Net) are invited to participate in the CRE25 survey, including all European Union (EU) and European Economic Area (EEA) countries, the Western Balkan countries (Albania, Bosnia and Herzegovina, Kosovo1, Montenegro, North Macedonia, Serbia) and Türkiye.
This analysis plan outlines the interim and final analysis for the CRE25 survey. It describes the baseline analysis to be conducted by ECDC which does not exclude additional and more in-depths analysis of specific findings of interest. The analysis plan is based on the previous CCRE survey analysis plan and takes into account the experience and lessons learned from the analyses of the EuSCAPE and CCRE survey data sets. Inclusion criteria, definitions and variables are described in the CRE25 survey protocol, as well as the data and isolate collection process for the CRE25 survey. The survey isolate collection period is from 1 October 2025 to 31 December 2025. Epidemiological and whole genome sequencing (WGS) data will be submitted by nominated country users via EpiPulse Cases using the AMRISO and AMRISO$AST data set, as described in the protocol.
