Fourth external quality assessment scheme for typing of verocytotoxin-producing E.coli (VTEC)
External quality assessment (EQA) schemes enable laboratories to assess their methods and procedures so that sources of systematic and random errors can be identified, eliminated, or reduced. Thus, an EQA also improves the accuracy of national and EU-level surveillance. This report presents the results of the fourth round of the VTEC external quality assessment scheme for the typing of verocytotoxin-producing E. coli (VTEC). The fourth EQA was carried out from November 2012 to February 2013 and included the following methods: pulsed-field gel electrophoresis (PFGE), O:H serotyping, detection of virulence genes (eae, vtx1, vtx2 and ehxA), subtyping of the vtx genes, phenotypic detection of verocytotoxin/Shiga toxin production (VT/Stx), fermentation of sorbitol, production of β-glucuronidase, enterohaemolysin, and extended β-lactamase (ESBL). Overall results were good. However, there are a few European laboratories which could benefit from a review of their in-house procedures and protocols.
Community engagement and institutional collaboration during outbreaks of Shiga toxin/verocytotoxin-producing Escherichia coli in Ireland
19 Nov 2019 - This country visit report presents the findings of a case study into outbreaks of Shiga toxin/verocytotoxin-producing Escherichia coli (STEC/VTEC) at childcare facilities in Ireland, conducted in November 2018.
Eighth external quality assessment scheme for typing of Shiga toxin-producing Escherichia coli
28 Feb 2019 - This report presents the results of the eighth round of the external quality assessment (EQA-8) scheme for typing of Shiga toxin-producing Escherichia coli (STEC) organised for laboratories providing data to the Food- and Waterborne Diseases and Zoonoses Network (FWD-Net) managed by the European Centre for Disease Prevention and Control (ECDC).