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This report presents the results of the 11th round of the external quality assessment (EQA-11) scheme for typing of Salmonella enterica subsp. enterica organised for the national public health national reference laboratories (NPHRLs) in ECDC’s Food- and Waterborne Diseases and Zoonoses network (FWD-Net) managed by the European Centre for Disease Prevention and Control (ECDC). The EQA-11 scheme was arranged by the Section for Foodborne Infections at the Statens Serum Institut (SSI) in Denmark.

Since 1 August 2019, a multi-country outbreak, caused by Salmonella Typhimurium ST19 and S. Anatum ST64, has affected three European Union (EU) countries, the United Kingdom and Canada.

​This joint rapid outbreak assessment from ECDC and EFSA assesses a cluster of monophasic Salmonella Typhimurium with an MLVA pattern previously unseen in the EU/EEA.

This report describes the standardised laboratory protocol for molecular subtyping of Salmonella enterica serotype Typhimurium. It is based on the 5-locus multiple-locus variable-number tandem repeat analysis (MLVA) method.